Composite
Ace

Part:BBa_K896987

Designed by: Hsieh Tien-Chan   Group: iGEM12_NYMU-Taipei   (2012-04-26)

This parts is artificially composed by Adonis Hsieh from Taipei, Taiwan

This part encodes a special Human potassium chloride cotransporter SLC12A4. With the combination of promoter BBa_J23100, this part can be modified when NheI and AvrII are presented.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 732
    Illegal BamHI site found at 2058
    Illegal XhoI site found at 220
    Illegal XhoI site found at 3245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 450
    Illegal NgoMIV site found at 2000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3041
    Illegal SapI site found at 901
    Illegal SapI.rc site found at 278


[edit]
Categories
Parameters
None